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Discovery Of Faulty Genes Could Reveal Risk Of Bone Disease
The discovery of faulty genes by Edinburgh researchers could help people with Paget"s disease, a painful bone condition. Dr Omar Albagha has found three genes associated with the disease which, if detected early enough in people, could hasten diagnosis and treatment.
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Document Examines Health Status Of Native American Indians Of Alabama
A newly published document, titled "Health Survey of American Indians of Alabama 2008:
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Republicans Test Public Plan Supporters' Will
Congressional Republicans are pushing an idea unlikely to garner much traction that would force members of Congress who vote for a government-run public plan for health insurance coverage to enroll in it, Politico reports. "Rep. John Fleming (R-La.), a family physician, kicked off the quixotic bid last week, urging House members to give up their right to participate in the much-revered Federal Employees Health Benefits Program if they support a government-run program as part of the health care reform package. Sens. John McCain of Arizona and Tom Coburn of Oklahoma are pushing the same concept in the Senate, preparing separate amendments that would require members - and maybe even their staffs - to sign up for the public option."
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Innovative Technology Shatters The Barriers Of Modern Light Microscopy

Researchers at the Helmholtz Zentrum MÃønchen and the Technische Universität MÃønchen are using a combination of light and ultrasound to visualize fluorescent proteins that are seated several centimeters deep into living tissue. In the past, even modern technologies have failed to produce high-resolution fluorescence images from this depth because of the strong scattering of light. In the Nature Photonics journal, the Munich researchers describe how they can reveal genetic expression within live fly larvae and fish by "listening to light". In the future this technology may facilitate the examination of tumors or coronary vessels in humans. Since the dawn of the microscope scientists have been using light to scrutinize thin sections of tissue to ascertain whether they are healthy or diseased or to investigate cell function. However, the penetration limits for this kind of examination lie between half a millimeter and one millimeter of tissue. In thicker layers light is diffused so strongly that all useful details are obscured. Together with his research team, Professor Vasilis Ntziachristos, director of the Institute of Biological and Medical Imaging of the Helmholtz Zentrum MÃønchen - German Research Center for Environmental Health and chair for biological imaging at the Technische Universität MÃønchen, has now broken through this barrier and rendered three-dimensional images through at least six millimeters of tissue, allowing whole-body visualization of adult zebra fish. To achieve this feat, Prof. Ntziachristos and his team made light audible. They illuminated the fish from multiple angles using flashes of laser light that are absorbed by fluorescent pigments in the tissue of the genetically modified fish. The fluorescent pigments absorb the light, a process that causes slight local increases temperature, which in turn result in tiny local volume expansions. This happens very quickly and creates small shock waves. In effect, the short laser pulse gives rise to an ultrasound wave that the researchers pick up with an ultrasound microphone. The real power of the technique, however, lies in specially developed mathematical formulas used to analyze the resulting acoustic patterns. An attached computer uses these formulas to evaluate and interpret the specific distortions caused by scales, muscles, bones and internal organs to generate a three-dimensional image. The result of this "multi-spectral opto-acoustic tomography", or MSOT, is an image with a striking spatial resolution better than 40 micrometers (four hundredths of a millimeter). And best of all, the sedated fish wakes up and recovers without harm following the procedure. Dr. Daniel Razansky, who played a pivotal role in developing the method, says, "This opens the door to a whole new universe of research. For the first time, biologists will be able to optically follow the development of organs, cellular function and genetic expression through several millimeters to centimeters of tissue." In the past, understanding the evolution of development or of disease required numerous animals to be sacrificed. With a plethora of fluorochrome pigments to choose from - including pigments using the fluorescence protein technology for which a Nobel Prize was awarded in 2008 and clinically approved fluorescent agents - observing metabolic and molecular processes in all kinds of living organisms, from fish to mice and humans, will be possible. The fruits of pharmaceutical research can also be harvested faster since the molecular effects of new treatments can be observed in the same animals over an extended period of time. Bio-engineer Ntziachristos is convinced that, "MSOT can truly revolutionize biomedical research, drug discovery and healthcare. Since MSOT allows optical and fluorescence imaging of tissue to a depth of several centimeters, it could become the method of choice for imaging cellular and subcellular processes throughout entire living tissues." Reference: Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo Daniel Razansky, Martin Distel, Claudio Vinegoni, Rui Ma, Norbert Perrimon, Reinhard W. Köster & Vasilis Ntziachristos Nature Photonics, published online on 21 June 2009; doi:10.1038/nphoton.2009.98 Sven Winkler Helmholtz Zentrum MÃønchen - German Research Center for Environmental Health


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