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Transition To Electronic Health Records Is Now Complete At Randolph Medical Center - Alabama Department Of Public Health
A successfully completed electronic health record pilot project at Randolph Medical Center in
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DFG To Support 6 New Interdisciplinary Research Units
During its summer session, the Joint Committee of the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) has approved funding for six new Research Units. Within these Research Units, researchers from different disciplines work together on a particular scientific topic. Funding for Research Units provides the personnel and equipment required for medium-term (usually six years) cooperation and contributes to the establishment of new research directions.
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ZyGEM Releases Data Confirming Its PrepGEM(R) Bacteria Kit Is A Simple, Universal Method For Extracting DNA From Different Bacteria Species
ZyGEM Corp. Ltd., a provider of innovative enzyme-based products for DNA extraction and other life sciences applications, today announced the availability of a new application note documenting that its prepGEM(R) Bacteria DNA extraction kit is capable of producing high quality, ready-to-analyze DNA from a broad range of bacterial species, including mixed or unknown sample types. This addresses an important unmet need --current DNA extraction methods typically require the use of different enzymes for each type of bacteria tested. The prepGEM(R) Bacteria kit also uses a single closed-tube system that reduces extraction time and cost while protecting the sample from contamination and making the kit easily adaptable for automation.
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The Characteristics Of Clarithromycin-Resistant Helicobacter pylori

Clarithromycin is currently one of the antibiotics used for eradication of Helicobacter pylori. However, reports of H. pylori resistance to this antibiotic are increasing worldwide. Clarithromycin resistance has been attributed to the presence of mutations in the 23S rRNA gene, a component of the ribosome that is the protein manufacturing machinery of all living cells. There is little information on the prevalence and characteristics of clarithromycin resistance in H. pylori strains isolated from Malaysian patients. In a research article published in the World Journal of Gastroenterology, the authors determined the prevalence of resistance and characterized the types of mutations present in their resistant strains. In this study conducted by Norazah et al on strains isolated in Malaysia, a low prevalence of clarithromycin-resistant strains was noted. Transitions of adenine to guanine at positions 2142 and 2143 of the 23S rRNA of H. pylori were the main mutations found in clarithromycin-resistant isolates. The A2142G and 2143G mutations of 23S rRNA genes in these strains can be detected easily by restriction fragment length polymorphism (RFLP) analysis of the PCR product of the 23S rRNA genes, using MboII or BsaI restriction enzymes. This eliminates the need to detect these mutations by sequencing. The result of the study demonstrates that clarithromycin resistance is present among local strains. However, even though it is an uncommon occurrence, the possibility of the patient being infected with a resistant strain should be considered if the patient does not respond to treatment with clarithromycin, so that an alternative treatment can be given. The ability to detect clarithromycin resistance by molecular methods such as RFLP showed that this rapid method has the potential to be used as a diagnostic tool. Reference: Ahmad N, Zakaria WR, Abdullah SA, Mohamed R. Characterization of clarithromycin resistance in Malaysian isolates of Helicobacter pylori. World Journal of Gastroenterology 2009; 15(25):3161-3165 http://www.wjgnet.com/1007-9327/15/3161.asp Correspondence to: Dr. Norazah Ahmad, Bacteriology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia. Lin Tian World Journal of Gastroenterology


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